Retro 2

Research use only, not for human use.

Retro 2 is a plant toxin ricin ?inhibitor, itprotects HeLa cells against Ricin, Stx1 and Stx2, selectively blocks retrograde toxin trafficking at the early endosome-TGN (trans-Golgi network) interface.

Retro 2 is a plant toxin ricin ?inhibitor, itprotects HeLa cells against Ricin, Stx1 and Stx2, selectively blocks retrograde toxin trafficking at the early endosome-TGN (trans-Golgi network) interface.

Retro-2 (1 μM; 1-4 hours) induces autophagy in GFP-LC3-expressing HeLa cells and promoted the dramatic cytoplasmic accumulation of large autophagosomes.Retro-2 (1 μM; 0.5-4 hours) treatment hows an increase over time of the abundance of LC3-II protein in cells.Retro-2 impairs the trafficking between autophagosomes and lysosomes. Retro-2 abolishes autolysosomes formation.Retro-2 (20 μM; pretreatment for 30 min) inhibits HeLa cell intoxication by ricin, Shiga-like toxins 1 (Stx1) and 2 (Stx2).Retro-2 blocked invasion of cells by the intracellular parasite Leishmania, as well as replication of non-enveloped viruses, including polyoma-, papilloma-, and adeno-associated viruses. Cell Autophagy Assay Cell Line:HeLa cells Concentration:1 μM Incubation Time:1 hour, 2 hours, 4 hours Result:Resulted in a large significant increase in the number of small and large vesicles.Western Blot Analysis Cell Line:HeLa cells Concentration:1 μM Incubation Time:0.5 hour, 2 hours, 4 hours Result:Showed an increase over time of the abundance of LC3-II protein in cells.

Retro-2 (2-200 mg/kg; i.p; daily; for 21 days) treatment clearly protects from lethal nasal exposure to ricin in mice. 200 mg/kg of Retro-2 fully protects mice against ricin challenge. Animal Model:Female Balb/c mice (Pathogen-free 6-week-old) injected with ricin (2 μg/kg)Dosage:2 mg/kg, 10 mg/kg, 20 mg/kg, 200 mg/kg Administration:Intraperitoneal injection; daily; for 21 days Result:Protected mice against ricin challenge.

2-{[(5-methyl-2-thienyl)methylene]amino}

Others

Other Targets

toxin ricin

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1201652-50-7

320.4

C19H16N2OS

>98% (HPLC)

DMSO:10 mM

CC1=CC=C(S1)C=NC2=CC=CC=C2C(=O)NC3=CC=CC=C3

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(-20℃)

With Ice Pack

≥ 2 years